tively characterize the total culturable subgingival-plaque flora of one dog with ligatures and one dog without. Thus, at numer- ous time points, plaque samples 

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Subgingival plaque samples (SPS) and stimulated saliva samples (SSS) were periodontal outcome, periodontitis, saliva sample, subgingival plaque sample 

2. 1. Oral Microbiol Immunol. 1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy.

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The subgingival plaque from each site was collected by placing the curette at the apical extent of the gingival crevice and drawing it coronally with slight pressure against the tooth surface. Curette sampling and a subgingival washing technique were compared within and between paired sites on two separate occasions in 24 patients using darkfield microscopy. Significantly lower mean proportions of spirochetes and motile rods and higher proportions of non-motile organisms were obtained with subgingival washing compared with curette sampling. There were no significant differences in Before and immediately after treatment, subgingival plaque samples were taken from interdental sites with 3 to 5 mm probing depth (PD) at 2 test teeth and 2 positive control teeth.

Subgingival plaque samples are also collected for microbiological analysis of the microbiota with focus on hydrogen sulfide producing bacteria. Samples are 

Aggregatibacter actinomycetemcomitans had been detected subgingivally in all prior to anti-infective therapy (AT) and METHODS: In 100 patients with aggressive/chronic periodontitis, subgingival plaque was sampled from the deepest pockets per quadrant (MT4) and per sextant (MT6). Plaque samples were taken using two sterile paper points simultaneously. One paper point from each pocket was pooled with the three other paper points of the pockets (MT4).

Subgingival plaque sampling

the subgingival microbiota, pathogens, and plaque [12]. In the present study, a gingival retraction cord (a specific dentistry tool) was used to sampling GCF and plaque: this is a novel sampling method that is potentially more reproducible and less technique-sensitive than the paper point sampling method.

Subgingival plaque sampling

Bacterial assays indicated that GCF strips removed significant numbers of bacteria when placed intracrevicularly for 5 s.

Subgingival plaque sampling

J Periodontol 2014 Jun;. Comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction. J Periodontol 2007  plaque and its significance in health and Lau CN, Levanos VA, et al. Bacterial diversity in human subgingival plaque. national sample survey population. Reproducibility of subgingival bacterial samples from patients with Effect of oxybenzone on PGE2-production in vitro and on plaque and gingivitis in vivo. Plaque samples were.
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Subgingival plaque sampling

to 3 p.m. 2. 1. Oral Microbiol Immunol.

Jens Kaltschmitt, Dr. med. dent.
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Previous studies have demonstrated that the hydrolysis of the trypsin substrate N-benzoyl-DL-arginine-2-naphthylamide (BANA), by subgingival plaque obtained from a single site, correlates best with the numbers and proportions of spirochetes in plaque samples and may serve as an indicator of clinical disease.

1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF. METHODS: In 50 patients with periodontitis, subgingival plaque was sampled from the deepest pocket of each quadrant by using paper points and by gaining saliva with saline mouthrinse. Analysis was performed using a commercially available polymerase chain reaction test for 11 periodontal pathogens.


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Before food reaches consumers it is checked by sampling and analysis. subgingival plaque have been attributed in part to the local availability of blood 

It is prepared from the roots, twigs and stem of Salvadora persica or othe Journal of Investigative and Clinical Dentistry (2010), 1, 126–132 ORIGINAL ARTICLE Oral Microbiology Detection rates of presumptive periodontal pathogens in subgingival plaque samples of untreated periodontitis using either four or six pooled samples Martin Wohlfeil, Orhan Tabakci, Rita Arndt, Peter Eickholz & Katrin Nickles Department of Periodontology, Center for Dental, Oral, and Two Subgingival Plaque-Sampling Strategies Used With RNA Probes. Autores: Diana-Maria Krigar Localización: Journal of periodontology, ISSN 0022-3492, Vol. 78, Nº. 1, 2007, págs. 72-78 Distribution of3-Hydroxy iC17:0 in Subgingival Plaque and Gingival Tissue Samples: Relationship to Adult Periodontitis FRANKC. NICHOLS* DepartmentofPeniodontology, University ofConnecticut Schoolof DentalMedicine, Farmnington, Connecticut 06030 Received 17 February 1994/Returned for modification 25 March 1994/Accepted 6 June 1994 removes sufficient bacteria to affect subsequent subgingival plaque sampling using a curette.

1. Oral Microbiol Immunol. 1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF.

Quantitative analysis of periodontal bacteria is considered useful for clinical diagnosis, evaluation and assessment of the risk of periodontal disease. BACKGROUND: The chewing stick, the miswak, is used in many developing countries as the traditional means for oral hygiene. It is prepared from the roots, twigs and stem of Salvadora persica or othe Journal of Investigative and Clinical Dentistry (2010), 1, 126–132 ORIGINAL ARTICLE Oral Microbiology Detection rates of presumptive periodontal pathogens in subgingival plaque samples of untreated periodontitis using either four or six pooled samples Martin Wohlfeil, Orhan Tabakci, Rita Arndt, Peter Eickholz & Katrin Nickles Department of Periodontology, Center for Dental, Oral, and Two Subgingival Plaque-Sampling Strategies Used With RNA Probes. Autores: Diana-Maria Krigar Localización: Journal of periodontology, ISSN 0022-3492, Vol. 78, Nº. 1, 2007, págs. 72-78 Distribution of3-Hydroxy iC17:0 in Subgingival Plaque and Gingival Tissue Samples: Relationship to Adult Periodontitis FRANKC. NICHOLS* DepartmentofPeniodontology, University ofConnecticut Schoolof DentalMedicine, Farmnington, Connecticut 06030 Received 17 February 1994/Returned for modification 25 March 1994/Accepted 6 June 1994 removes sufficient bacteria to affect subsequent subgingival plaque sampling using a curette. In 25 subjects, one healthy, gingivitis and periodontitis site was  subgingival biofilm sampling by paper point and curette.

Abstract. Background: The collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. However, the collection of saliva  22 Mar 2017 Material and methods: Plaque samples of 50 patients with generalized severe chronic periodontitis before therapy were pooled in two separate  Supragingival plaque should be removed with cotton pellets or periodontal scalers to secure noncontaminated subgingival specimens. Blood and pus at the   gingivalis in saliva and in subgingival plaque samples, showing comparable specificity to culture and real-time PCR. Conclusion: We applied the FRET technology  2 Jan 2018 DNA was then extracted from plaque samples and analyzed by 16S.